Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-89 86
EDTA plasma(n=5) 79-95 80
heparin plasma(n=5) 80-94 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-102% 97-105% 95-102% 93-101%
EDTA plasma(n=5) 92-99% 83-99% 78-101% 92-99%
heparin plasma(n=5) 83-96% 79-97% 84-98% 80-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Pentraxin 3, Long (PTX3) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis ModelPubMed: 22966213
  • Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand RabbitsSfu: Source
  • Hemodialysis-induced changes in the blood composition affect function of the endotheliumOnlinelibrary: hdi.12148
  • Hemodialysis‐induced changes in the blood composition affect function of the endotheliumPubmed:24612443
  • The effects of sunitinib on endometriosisPubmed:25093747
  • Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic ratsPubmed:25366063
  • Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3Pubmed:25510691
  • Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parametersPubMed: 26223286
  • The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat modelPubMed: 26470829
  • Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers5952149
  • Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat ModelPubmed:29725602
  • The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart DiseasepaperID:86206
  • Serum levels and gene expression of pentraxin 3 are elevated in COPDPubmed: 30572222
  • Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C
  • Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation
  • Study on Serum Pentraxin-3 Levels in Vasculitis with HypertensionPubmed: 31264920
  • The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitisPubmed: 31798002
  • Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental studyPubmed: 31955629
  • Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat ModelPubmed: 32593749
  • Therapeutic effects of vitamin D on acetic acid-induced colitis in ratsPubmed: 32555936
  • Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children33716459
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
  • Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus34595798

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