Multiplex Assay Kit for Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array)

Matrices listed below were spiked with certain level of recombinant Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Anti-Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) and unlabeled Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) ,etc. by CBA (Cytometric Bead Array) in the sample.