Multiplex Assay Kit for Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) 
SLC6A4; SLC6-A4; HTT; 5-HTT; 5HTT; OCD1; hSERT; Solute Carrier Family 6,Member 4; Neurotransmitter Transporter,Serotonin; Sodium-dependent serotonin transporter
Overview
Properties
- Product No.CMC890Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryNeuro science
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-99 | 93 |
| EDTA plasma(n=5) | 78-99 | 90 |
| heparin plasma(n=5) | 93-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-101% | 87-95% | 86-101% | 78-89% |
| EDTA plasma(n=5) | 92-101% | 81-93% | 97-105% | 92-101% |
| heparin plasma(n=5) | 97-105% | 81-93% | 92-101% | 89-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Serotonin Transporter (SERT) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and controlPubmed:25127373
- Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brainPubmed:25400562
- Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjectsPubMed: 26527205
- Non-Serotonergic Neurotoxicity by MDMA (Ecstasy) in Neurons Derived fromMouse P19 Embryonal Carcinoma Cells.pubmed:27861613
- Herz-Hirn Interaktion im Mausmodell: Herzinsuffizienz nach Myokardinfarkt führt zu depressivem Verhalten bei Mäusenfrontdoor:index
- Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Ratsarticle_1486_c0fa9f8afe5f70b407e7195bcfd55c70
- Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …Pubmed:29854627
- Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
- Effects of regulating gut microbiota on the serotonin metabolism in the chronic unpredictable mild stress rat modelPubmed: 31323174
- VAMP8 and serotonin transporter levels are associated with venous thrombosis risk in a Spanish female population. Results from the RETROVE ProjectPubmed: 31382081
- Neuronal Cell Death Induces Depressive Disorder in Rats Depression-Like Behaviors Caused by Chronic Stress
- Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats34568500
- Targeting the oxytocin system to ameliorate early life depressive-like behaviors in maternally-separated rats34349049