Multiplex Assay Kit for Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) 
ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin
Overview
Properties
- Product No.CMA396Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyReproductive science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 96 |
| EDTA plasma(n=5) | 83-96 | 89 |
| heparin plasma(n=5) | 87-97 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 95-102% | 79-96% | 90-104% |
| EDTA plasma(n=5) | 85-103% | 80-102% | 87-101% | 81-97% |
| heparin plasma(n=5) | 84-103% | 85-103% | 80-93% | 80-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
Increment services
Citations
- A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor BiomarkersTandfonline: Source
- Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
- Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric SyndromeSMGHBM_2016_v26n9_1063
- Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
- Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
- Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male ratspubmed:27746313
- 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响yycyzx201733003
- Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
- Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis34029952
- Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats33868755
- Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459