Multiplex Assay Kit for Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 393.00 US$ 562.00
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  • Multiplex Assay Kit for Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 99
EDTA plasma(n=5) 78-93 88
heparin plasma(n=5) 79-93 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-94% 94-102% 90-97% 80-102%
EDTA plasma(n=5) 82-98% 83-96% 87-95% 89-99%
heparin plasma(n=5) 89-102% 93-101% 78-98% 93-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Stromal Cell Derived Factor 1 (SDF1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)Goescholar: source
  • The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vesselsScienceDirect: S0142961209012654
  • The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanismsScienceDirect: S0142961211011422
  • Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia modelScienceDirect: S0944711310003260
  • Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cellsPubMed: 21655971
  • Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemiaPubMed: 21224834
  • Elevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with PolyangiitisPubMed: 22792461
  • Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cellsPubmed: 24780853
  • The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trialPubmed:24530788
  • Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary menPubmed:24286591
  • Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model RatPubmed:24814095
  • Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depressionPubmed:25150002
  • Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke PatientsPubmed:25444027
  • The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disordersPubmed:25560580
  • Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cellsPubmed:25636237
  • Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorderPubMed: 26481549
  • Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic ratsPubMed: 26392311
  • Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive ratsPubMed: 26396666
  • A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural EffusionPubMed: 26632727
  • A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidityPubmed:27208878
  • Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.pubmed:28149283
  • High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia.pubmed:27761657
  • AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungspubmed:29233725
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancerpubmed:28573141
  • Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.pubmed:28842629
  • Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study.pubmed:29017079
  • The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegrationPubmed:29850672
  • Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expressionPubmed:29620151
  • β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive ratsPubmed:29752864
  • Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantationDoi: 10.1016/j.jcyt.2018.07.007
  • Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone RegenerationPubmed: 30777748
  • Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cellsPubmed: 30949297
  • Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem CellsPubmed: 31827537
  • Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callusPubmed: 32894200
  • Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4Pubmed: 32633015
  • Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia33878473
  • bFGF and SDF-1¦Á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts33800631
  • Combination of an engineered Lactococcus lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice34310856

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