Multiplex Assay Kit for Substance P (SP) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 385.00 US$ 550.00
  • Quantity
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  • Multiplex Assay Kit for Substance P (SP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Substance P (SP) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Substance P (SP) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Substance P (SP) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-105 101
EDTA plasma(n=5) 93-101 96
heparin plasma(n=5) 98-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Substance P (SP) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Substance P (SP) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Substance P (SP) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-92% 81-91% 86-95% 79-96%
EDTA plasma(n=5) 80-105% 78-102% 78-101% 86-101%
heparin plasma(n=5) 83-92% 90-99% 93-105% 85-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Substance P (SP) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Substance P (SP) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Substance P (SP) ,etc. by CBA (Cytometric Bead Array) and unlabeled Substance P (SP) ,etc. by CBA (Cytometric Bead Array) (Standards or samples) with the pre-coated antibody specific to Substance P (SP) ,etc. by CBA (Cytometric Bead Array). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Substance P (SP) ,etc. by CBA (Cytometric Bead Array) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Substance P (SP) ,etc. by CBA (Cytometric Bead Array) in the sample.

Giveaways

Increment services

Citations

  • A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic PainPubmed: 23659719
  • Oral curcumin has anti-arthritic efficacy through somatostatin generationPubMed: 25836921
  • Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
  • Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulationPubmed:29900692
  • Serum Levels of Neuropeptides in Cows with Left Abomasal DisplacementPubmed: 30562932
  • Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455
  • Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine modelPubmed: 33014123
  • Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy
  • Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis34213714
  • Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice34626771

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