Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

CD138; SDC; SYND1; Syndecan Proteoglycan 1

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  • Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-95 91
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 79-89 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-102% 81-93% 83-97% 92-102%
EDTA plasma(n=5) 98-105% 97-104% 99-105% 80-88%
heparin plasma(n=5) 94-101% 87-95% 86-96% 81-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Syndecan 1 (SDC1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarctionSpringerLink: a201w3l18k257462
  • Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI RatsSpringer: Source
  • Damage of the endothelial glycocalyx in chronic kidney diseasePubmed:24727235
  • Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injuryPubmed:25058256
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx SheddingPubMed: 26199464
  • Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.PubMed: 26371596
  • Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injurypubmed:27405618
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats10.1089
  • Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.doi/10.1089/neu.2016.4574
  • Volume kinetics of Ringer's lactate solution in acute inflammatory disease10.1016:j.bja.2018.04.023
  • MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic chargePubmed:29903730
  • Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsisPubmed:28591008
  • Experimental models of endotheliopathy: impact of shock severityPubmed:28697004
  • Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the ratPubmed:28930945
  • The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions173015_06.pdf
  • Plasma ameliorates endothelial dysfunction in burn injuryDoi: 10.1016/j.jss.2018.08.027
  • Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke modelPubmed: 30374889
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuitPubmed: 31787433
  • Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICPPubmed: 31919038
  • Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
  • Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis33375342
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832
  • Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke34524269
  • Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock modelPubmed:35110424
  • Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase

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