Multiplex Assay Kit for Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) Mus musculus (Mouse) Protein, Antibody, Assaykit, Service

CD141; THBD; BDCA3; THRM; Fetomodulin

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 452.00 US$ 646.00
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  • Multiplex Assay Kit for Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-104 87
EDTA plasma(n=5) 83-105 95
heparin plasma(n=5) 80-92 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 91-99% 95-102% 90-104%
EDTA plasma(n=5) 98-105% 94-101% 96-105% 95-104%
heparin plasma(n=5) 87-102% 94-103% 83-97% 85-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Thrombomodulin (TM) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • A Non-Lethal Traumatic/Hemorrhagic Insult Strongly Modulates the Compartment-Specific PAI-1 Response in the Subsequent Polymicrobial SepsisPubMed: PMC3568129
  • Is There Any Association Between Thrombosis and Tissue Factor Pathway Inhibitor Levels in Patients With Vitamin D Deficiency?Pubmed: 24203351
  • КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В …Ru:Source
  • Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in micePubmed:24451386
  • The effect of hormone therapy on biochemical and ultrasound parameters associated with atherosclerosis in 46, XY DSD individuals with female phenotypePubmed:24911331
  • Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in ratsPubmed:25109347
  • Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleenPubmed:25243628
  • Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder InjuryPubMed: 26351640
  • Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
  • Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture studyPubMed: 26689470
  • КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В СОЧЕТАНИИ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙCozo: Mos
  • Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture studyPubmed:26689470
  • The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective functionand prevents SOS in a murine model.pubmed:27427915
  • Blood markers of fibrinolysis and endothelial activation in canine babesiosis.pubmed:28363279
  • Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domainPubmed:29903766
  • Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cellsPubmed:29775893
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407

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