Multiplex Assay Kit for Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) 
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Overview
Properties
- Product No.CMA524Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryHematologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-99 | 83 |
| EDTA plasma(n=5) | 93-101 | 97 |
| heparin plasma(n=5) | 89-101 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-101% | 84-92% | 98-105% | 79-98% |
| EDTA plasma(n=5) | 98-105% | 94-101% | 96-104% | 95-102% |
| heparin plasma(n=5) | 87-101% | 78-94% | 79-92% | 89-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Tissue Factor (TF) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.
Giveaways
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Citations
- Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive ratsPubMed: 18625276
- Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factorNrcresearchpress: y10-115
- Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in ratsPubmed: 23538027
- Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformationsPubmed: 24199192
- Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during PregnancyPubmed:25505239
- Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway InhibitorPubMed: 20011089
- Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-ShockPubMed: 25423126
- Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
- Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
- The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
- Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injurypubmed:29202212
- Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndromePubmed: 29202212
- Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in ratsPubmed: 31755229
- Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB …Pubmed: 32587471
- Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation SystemPubmed:35685509