Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) Canis familiaris; Canine (Dog) Protein, Antibody, Assaykit, Service

HITF; ITF3; TFI; HP1.B; Trefoil Factor 3, Intestinal

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 669.00 US$ 955.00
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  • Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-104 98
EDTA plasma(n=5) 87-99 92
heparin plasma(n=5) 96-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 80-105% 87-105% 84-98%
EDTA plasma(n=5) 85-102% 83-101% 80-101% 99-105%
heparin plasma(n=5) 80-88% 87-104% 83-101% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Trefoil Factor 3 (TFF3) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in ChinaBiomedcentral: Source
  • Serum Trefoil Factor 3 as a Protein Biomarker for the Diagnosis of Colorectal Cancer.pubmed:27760866
  • Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition.pubmed:29028798
  • Protective effects of sinomenine against LPS-induced inflammation in piglets.pubmed:28757275
  • Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning processPubmed:29555969
  • Kidney-based in vivo model for drug-induced nephrotoxicity testingPubmed: 32796873
  • Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
  • Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­

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