Multiplex Assay Kit for Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) Protein, Antibody, Assaykit, Service

TUB-B; TUBB5; TUBB1; M40

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 575.00 US$ 821.00
  • Quantity
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  • Multiplex Assay Kit for Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-99 92
EDTA plasma(n=5) 79-90 84
heparin plasma(n=5) 80-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-96% 90-104% 95-102% 96-104%
EDTA plasma(n=5) 93-101% 84-94% 99-105% 98-105%
heparin plasma(n=5) 95-102% 86-99% 89-99% 93-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Tubulin Beta (TUBb) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analoguespubmed:27747473
  • New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition.pubmed:28150327
  • Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixturepubmed:28112513
  • Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agentsPubmed:29779400
  • Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerizationPubmed:29966433
  • Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
  • Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2InhibitorsPubmed: 30191744
  • Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking studyPubmed: 31536891
  • Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platformPubmed: 32207606
  • Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their …Pubmed: 32325332
  • Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in ratsPubmed: 33028437
  • Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon …Pubmed: 33158579
  • Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells33300466
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026

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