Multiplex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) Protein, Antibody, Assaykit, Service

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

  • UOM
    1. 48T
    2. 96T
  • FOB US$ 605.00 US$ 864.00
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  • Multiplex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Multiplex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-99 94
EDTA plasma(n=5) 90-102 98
heparin plasma(n=5) 90-99 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-88% 94-102% 92-101% 84-99%
EDTA plasma(n=5) 78-99% 78-93% 99-105% 88-101%
heparin plasma(n=5) 80-94% 82-103% 91-105% 80-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

Multiplex Assay Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array)

Test principle

Anti-Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) antibody was coated on the surface of magnetic beads, to make solid phase carrier. Standard or sample were added to the magnetic beads, respectively, in which Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) was bound to the antibody connected to the solid phase carrier, and then biotinylated anti-Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) antibodies were added. PE-labeled avidin was added. There is a positive correlation between the concentration of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ,etc. by CBA (Cytometric Bead Array) in the sample and corresponding value of MFI (Median Fluorescence Intensity). Flow Cytometer was used to determine and calculate the sample concentration.

Giveaways

Citations

  • Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesionsPubMed: 26232084
  • Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcomePubmed:26810533
  • Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135
  • Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytespubmed:28667290
  • Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disabilityPubmed:29523900
  • Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injuryarticle:10.1088
  • Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhageISBN:978-952-03-0750-9
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head TraumaPubmed: 32987792

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