OneStep ELISA Kit for Beta-2-Microglobulin (b2M) 
BMG; B2MG
Overview
Properties
- Product No.OEA260Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityKidney biomarker
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Beta-2-Microglobulin (b2M) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-2-Microglobulin (b2M) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-102 | 97 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 78-98 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-2-Microglobulin (b2M) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 78-101% | 94-101% | 78-94% |
| EDTA plasma(n=5) | 88-103% | 85-101% | 88-99% | 80-89% |
| heparin plasma(n=5) | 80-89% | 78-96% | 89-96% | 78-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microplate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to Beta-2-Microglobulin (b2M). After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M) and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
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Citations
- The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
- Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recoveryBiomedcentral: Source
- Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner.pubmed:28959042
- Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
- Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approachPubmed:29315472
- Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
- Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
- iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma33540066
- Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 146434573096
- Biomarkers of cadmium exposure and renal function in estuarine adult villagers34773507