OneStep ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Beta-Thromboglobulin

  • UOM
    1. 48T
    2. 96T
  • FOB US$ US$
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  • OneStep ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Packages (Simulation)
  • OneStep ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Chemokine (C-X-C motif) ligand 7 ( CXCL7) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-95 89
EDTA plasma(n=5) 90-97 93
heparin plasma(n=5) 92-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C motif) ligand 7 ( CXCL7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-102% 95-102% 90-104% 78-91%
EDTA plasma(n=5) 85-96% 85-93% 79-101% 78-98%
heparin plasma(n=5) 82-90% 82-101% 87-95% 89-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.

OneStep ELISA Kit for Chemokine (C-X-C motif) ligand 7 ( CXCL7)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C motif) ligand 7 ( CXCL7). Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to Chemokine (C-X-C motif) ligand 7 ( CXCL7). After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C motif) ligand 7 ( CXCL7) and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine (C-X-C motif) ligand 7 ( CXCL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Strict heart rate control attenuates prothrombotic state and platelet activity in patients with non-valvular permanent atrial fibrillation.Pubmed: 2347822
  • STUDIES ON THE STORAGE OF POOLED PLATELETS IN NON DOP PVC CONTAINERSEbscohost: Source
  • Bioprofiling of platelets in medicated patients with depression.Pubmed:25451399
  • Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
  • Differential changes in platelet reactivity induced by acute physical compared to persistent mental stressPubMed: 26192713
  • Bioprofiling of platelets in medicated patients with depressionPubMed: 25451399
  • Markers of coagulation and fibrinolysis do not detect or predict the presence of left atrial appendage thrombus in patients with atrial fibrillationPubmed: 32147650
  • A novel di‐peptide Met‐Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq‐mediated signalingPubmed: 32662128
  • Endogenous Nitric Oxide-Releasing Microgel Coating Prevents Clot Formation on Oxygenator Fibers Exposed to In Vitro Blood FlowPubmed:35054599
  • The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female ratsPubmed:35183388

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