OneStep ELISA Kit for Desmocollin 1 (DSC1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmocollin 1 (DSC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmocollin 1 (DSC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microplate provided in this kit has been pre-coated with an antibody specific to Desmocollin 1 (DSC1). Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to Desmocollin 1 (DSC1). After TMB substrate solution is added, only those wells that contain Desmocollin 1 (DSC1) and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmocollin 1 (DSC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.