OneStep ELISA Kit for Follicle Stimulating Hormone Beta (FSHb) 
FSH-B; Follitropin Subunit Beta
Overview
Properties
- Product No.OED017Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryReproductive science
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Follicle Stimulating Hormone Beta (FSHb) and the recovery rates were calculated by comparing the measured value to the expected amount of Follicle Stimulating Hormone Beta (FSHb) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 79-103 | 91 |
| heparin plasma(n=5) | 95-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone Beta (FSHb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone Beta (FSHb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follicle Stimulating Hormone Beta (FSHb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-90% | 95-102% | 94-101% | 83-93% |
| EDTA plasma(n=5) | 82-103% | 80-88% | 80-101% | 89-99% |
| heparin plasma(n=5) | 95-103% | 85-99% | 85-94% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Follicle Stimulating Hormone Beta (FSHb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between HRP labeled Follicle Stimulating Hormone Beta (FSHb) and unlabeled Follicle Stimulating Hormone Beta (FSHb) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone Beta (FSHb). After incubation, the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone Beta (FSHb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follicle Stimulating Hormone Beta (FSHb) in the sample.
