OneStep ELISA Kit for Tissue Factor (TF) 
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Overview
Properties
- Product No.OEA524Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryHematologyCardiovascular biology
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Tissue Factor (TF) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor (TF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| sodium citrate plasma(n=5) | 80-92 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor (TF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 90-103% | 80-101% | 96-103% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF). Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to Tissue Factor (TF). After TMB substrate solution is added, only those wells that contain Tissue Factor (TF) and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Factor (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
Increment services
Citations
- Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive ratsPubMed: 18625276
- Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factorNrcresearchpress: y10-115
- Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in ratsPubmed: 23538027
- Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformationsPubmed: 24199192
- Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during PregnancyPubmed:25505239
- Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway InhibitorPubMed: 20011089
- Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-ShockPubMed: 25423126
- Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
- Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
- The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
- Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injurypubmed:29202212
- Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndromePubmed: 29202212
- Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in ratsPubmed: 31755229
- Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB …Pubmed: 32587471
- Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation SystemPubmed:35685509