OneStep ELISA Kit for Vitamin D Binding Protein (DBP) 
GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
Overview
Properties
- Product No.OEB810Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayTumor immunity
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Vitamin D Binding Protein (DBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin D Binding Protein (DBP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-99 | 94 |
| EDTA plasma(n=5) | 82-95 | 90 |
| heparin plasma(n=5) | 95-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin D Binding Protein (DBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-93% | 90-98% | 98-105% | 98-105% |
| EDTA plasma(n=5) | 91-101% | 79-96% | 93-101% | 81-93% |
| heparin plasma(n=5) | 87-94% | 92-105% | 91-98% | 84-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microplate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microplate wells with a HRP-conjugated antibody specific to Vitamin D Binding Protein (DBP). After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP) and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
Increment services
Citations
- Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patientsPubMed: 23101585
- Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
- Vitamin D-binding protein and free vitamin D concentrations in acromegalyPubMed: 26547217
- Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
- 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients.pubmed:28085957
- Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
- 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients10.1371/journal.pone.0170323
- Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …Pubmed:30037068
- Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Pubmed: 30553018
- Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children?34013709
- Leukocyte telomere length as a compensatory mechanism in vitamin D metabolismPubmed:35202418