Wide-range ELISA Kit for Klotho (KL) 
KLa; Klotho Alpha
- UOM
- FOB US$ 479.00 US$ 684.00 US$ 3,078.00 US$ 5,814.00 US$ 47,880.00
- Quantity
Overview
Properties
- Product No.WEH757Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range Klotho (KL) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Klotho (KL) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-105 | 96 |
| EDTA plasma(n=5) | 94-101 | 98 |
| heparin plasma(n=5) | 79-93 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Klotho (KL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Klotho (KL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Klotho (KL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 97-105% | 90-102% | 80-97% |
| EDTA plasma(n=5) | 85-92% | 93-101% | 99-105% | 99-105% |
| heparin plasma(n=5) | 80-99% | 97-104% | 88-103% | 80-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Klotho (KL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Klotho (KL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Klotho (KL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculaturePubmed: 24012609
- Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in MiceCom:Source
- α-Klotho is an acute phase protein and altered by restraint stress in micePubmed:Pmc4203207
- Les biomarqueurs des calcifications vasculairesPubMed: 26069069
- Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with KlothoPubMed: 25873020
- Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3PubMed: 25899975
- 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制Cn: Article
- The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanismPubMed: 26419931
- Soluble Klotho protein as a novel serum biomarker in patients with acromegalypmc:PMC4754356
- Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O PathwayPubmed:27174564
- Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice.pubmed:28387374
- Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management?pubmed:28429560
- Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patientsissn:2394-2916
- Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in miceISSN:1936-2625
- Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat modelpubmed:28943597
- Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion InjuryPubmed:29550818
- Advanced maternal age impairs spatial learning capacity in young adult mouse offspringPubmed:29636887
- Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complicationsPubmed:30042059
- Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal …Pubmed:30004089
- High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney diseasePubmed: 30189026
- The role of klotho in chronic kidney diseasePubmed: 30348110
- Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regenerationPubmed: 30451844
- Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndromePubmed: 30770217
- The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic ratsPubmed: 30801205
- CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
- Effects of salt intervention on serum levels of Klotho influenced by salt sensitivityPubmed: 33164306
- Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic ratsPubmed: 32744464
- Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic ratsPubmed: 32438076
- P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy.Pubmed: 32377919
- Ameliorating Effect of Klotho Protein on Rat Heart during I/R InjuryPubmed: 33123314
- Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With SchizophreniaPubmed: 32612508
- ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy33675964
- Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma CellsPubmed:35112226
- Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
- Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome