Wide-range ELISA Kit for Lipoprotein lipase (LPL) Mus musculus (Mouse) Wide-range ELISA

LIPD; Lipase, Lipoprotein

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  • Wide-range ELISA Kit for Lipoprotein lipase (LPL) Packages (Simulation)
  • Wide-range ELISA Kit for Lipoprotein lipase (LPL) Packages (Simulation)
  • Wide-range ELISA Kit for Lipoprotein lipase (LPL) Results demonstration
  • WEA386Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Wide-range Lipoprotein lipase (LPL) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Lipoprotein lipase (LPL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-93 87
EDTA plasma(n=5) 83-101 86
heparin plasma(n=5) 88-104 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Lipoprotein lipase (LPL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Lipoprotein lipase (LPL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Lipoprotein lipase (LPL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 87-104% 84-102% 88-104%
EDTA plasma(n=5) 85-94% 86-93% 92-105% 96-105%
heparin plasma(n=5) 81-102% 78-97% 92-101% 78-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Wide-range ELISA Kit for Lipoprotein lipase (LPL)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Lipoprotein lipase (LPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Lipoprotein lipase (LPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Lipoprotein lipase (LPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Lipoprotein lipase (LPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Severity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and DisassemblyPubMed: 21646389
  • Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevationPubmed:27243230
  • ReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult MicePubmed:27234787
  • Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet10.1016/j.jff.2017.07.020
  • Mass Spectrometric Evaluation of Upstream and Downstream Process Influences on Host Cell Protein Patterns in Biopharmaceutical ProductsPubmed: 30767403
  • Integrin β3 Deficiency Results in Hypertriglyceridemia Via Disrupting LPL (Lipoprotein Lipase) SecretionPubmed: 32237906
  • Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial34024503
  • Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory …Pubmed:35204215

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