Adenylate Kinase 8 (AK8)

AK8 is the second known human AK with two complete and active AK domains within its polypeptide chain, a feature that previously was shown also for AK5.

The full length AK8, as well as its two domains AK8p1 and AK8p2, all showed similar AK enzyme activity. AK7, full length AK8, AK8p1 and AK8p2 phosphorylated AMP, CMP, dAMP and dCMP with ATP as phosphate donor and also AMP, CMP and dCMP with GTP as phosphate donor. Both AK7 and the full length AK8 showed highest affinity for AMP with ATP as phosphate donor and proved to be more efficient in AMP phosphorylation as compared to the major cytosolic isoform AK1. Expression of the proteins fused with the green fluorescent protein demonstrated a cytosolic localization for both AK7 and AK8.