Alkaline Phosphatase, Intestinal (ALPI)

Alkaline phosphatase, intestinal also known as ALPI is a type of alkaline phosphatase.The deduced polypeptide showed 86.5% amino acid identity to placental ALP and 56.6% amino acid identity to liver/bone/kidney ALP. Thus, the immunologic cross-reactivity of ALPI and ALPP is perhaps explained.

Calf Intestinal Alkaline Phosphatase (CIP) is an enzyme that catalyzes the 5' phosphate group's removal from DNA. This enzyme is frequently used in DNA sub-cloning, because DNA fragments which lack the 5' phosphoryl termini cannot self-ligate. Preventing self ligation is important both in improving the yield of properly ligated product, and reducing the background of improperly self-ligated contaminant.