Chromatin Modifying Protein 7 (CHMP7)

CHMP7 interacted with CHMP4B, but not with EAP20 (VPS25). Overexpression of fluorescence-tagged CHMP7 impaired transport of endocytosed EGF in the endosome-lysosome pathway. Moreover, overexpression of CHMP7 suppressed release of virus-like particles into the culture medium of cotransfected cells.

The deduced 453-amino acid protein contains an SNF7 (CHMP4A)-related domain with a coiled-coil region in its N-terminal half and an SNF7 domain with 2 coiled-coil regions in its C-terminal half. The N-terminal half of CHMP7 also has 2 putative calmodulin-binding IQ motifs and a clathrin-binding motif. Epitope-tagged CHMP7 showed diffuse cytoplasmic staining and a punctate pattern, especially in the perinuclear area.