Centlein, Centrosomal Protein (CNTLN)

Makino et al. (2008) used ion exchange chromatography and tandem mass spectrometry to study components of isolated centrosomes that cosedimented with microtubules. They identified one of these components as a 721-amino acid protein, called centlein, in rat brain.

The protein contains coiled-coil domains. Western blot analysis detected an approximately 90-kD protein in all adult rat tissues tested and in various cell lines. Transfection experiments in HeLa cells demonstrated that centlein colocalizes with the centrosomal protein gamma-tubulin. Scott (2008) mapped the CNTLN gene to chromosome 9p22.2 based on an alignment of the CNTLN sequence with the genomic sequence.