Chromodomain Helicase DNA Binding Protein 8 (CHD8)

By sequencing clones from a size-fractionated fetal brain cDNA library, Nagase et al. (2000) cloned CHD8, which they designated KIAA1564. The deduced protein contains 1,417 amino acids. RT-PCR ELISA detected moderate expression in all adult and fetal tissues and specific brain regions examined.

Ishihara et al. (2006) cloned mouse Chd8. The deduced 2,582-amino acid protein has 2 chromodomains, followed by a central helicase/ATPase domain and 2 C-terminal BRK domains. Northern blot analysis detected ubiquitous Chd8 expression in mouse tissues, with highest levels in heart and testis. Western blot analysis of HeLa cells detected endogenous CHD8 at an apparent molecular mass of 240 kD.