Epsin 1 (EPN1)

EPN1 is an endocytic accessory protein that interacts with EPS15, the alpha subunit of the clathrin adaptor AP2 (AP2A1), and clathrin, as well as with other accessory proteins for the endocytosis of clathrin-coated vesicles

The deduced 576-amino acid protein contains several conserved asp-pro-trp (DPW) repeats in its central region, a Cdc2 phosphorylation site, and 3 arg-pro-phe (NPF) repeats in its C-terminal region. Northern blot analysis detected a 2.6-kb transcript in all rat tissues tested, and Western blot analysis confirmed ubiquitous expression. Immunofluorescence localization using rat brain frozen sections revealed colocalization of Epn1 with Eps15, synaptophysin, and clathrin in nerve terminals.