Eukaryotic Translation Initiation Factor 1A Domain Containing Protein (EIF1AD)

Haponin (eIF1AD) interacts with glyceraldehyde 3-phosphate dehydrogenase in the CHO-K1 cell line. Haponin (HLDF-alike protein) was previously identified from the human promyelocytic leukemia HL-60 cell line. recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system.

Antibodies against 6xHis-haponin were produced, and the expression of endogenous haponin was demonstrated in mammalian cell lines of different origin. Using affinity chromatography and immunoprecipitation methods, in CHO-K1 cells haponin interacts with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes with a diverse set of noncanonical functions.