Interleukin 1 Delta (FIL1d)

PCR analysis revealed expression in leukocytes, spleen, and brain as well as in fetal brain and most abundantly in a fetal skin library. RT-PCR analysis also established that IL1HY1 expression is amplified in a stimulated macrophage cell line.

By EST database searching, Smith et al. (2000) also identified a cDNA encoding IL1HY1, which they termed FIL1-delta. Modeling indicated that IL1HY1 has a conserved 12-stranded beta-trefoil structure. By searching EST databases for IL1-like ligands, Busfield et al. (2000) also cloned IL1HY1, which they designated IL1RP3. SDS-PAGE analysis indicated that IL1HY1 is expressed as a 23-kD protein. Northern blot analysis detected expression of a 2.8-kb transcript in placenta and uterus. In situ hybridization analysis revealed constitutive expression by keratinocytes in vitro.