Midline 2 (MID2)

Midline-2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

MID1 and MID2 display 84% similarity and 77% identity at the protein level, and 70% identity at the nucleotide level. Like MID1, MID2 displays a RING finger, 2 B-boxes, a coiled-coil, and a conserved RFP-like C-terminal domain. The mouse Mid2 protein shows 99.3% identity with human MID2. MID2 appears to be part of a linkage group conserved between mouse and man.