Mitogen Activated Protein Kinase 10 (MAPK10)

Gupta et al. (1996) isolated brain cDNAs encoding 10 different JNK isoforms, 8 of which were derived from either JNK1  or JNK2 . The other 2 cDNAs were from a gene that the authors designated JNK3. JNK3 contains an extended N-terminal region not found in JNK1 or JNK2. The 2 JNK3 isoforms, called JNK3-alpha-1 and JNK3-alpha-2, have different C termini.

By SDS-PAGE of in vitro transcription/translation products, Gupta et al. (1996) determined that JNK3-alpha-1 migrates as a 45-to-48-kD doublet and JNK3-alpha-2 migrates as a 54-to-57-kD doublet. They stated that the lower band probably represents translation from a second in-frame start codon that corresponds to the first codon in JNK1 and JNK2. All the JNKs were activated by treatment of cells with the inflammatory cytokine IL1 .