Nucleoporin 160 (NUP160)

NUP160 is 1 of up to 60 proteins that make up the 120-MD nuclear pore complex, which mediates nucleoplasmic transport. By sequencing clones obtained from a size-fractionated myeloid cell line cDNA library, cloned NUP160. The deduced 1,314-amino acid protein contains a histidine acid phosphatase motif. Northern blot analysis revealed moderate expression in all tissues examined. Nup160 as a protein in Xenopus egg extracts that interacts with the nuclear pore basket proteins Nup98 and Nup153.Peptide analysis revealed homology with KIAA0197. Antibody raised against the putative human protein detected endogenous NUP160 in puncta along the nuclear rim in HeLa cells, and differential permeabilization of HeLa cells indicated that NUP160 is localized to the basket side of the pore, facing the nucleoplasm.