Prostate Cancer Susceptibility Candidate (PRAC)
C17orf92; Prostate,Rectum And Colon; Small nuclear protein PRAC1; Prostate cancer susceptibility candidate protein 1; Prostate, rectum and colon expressed gene protein
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Using these EST sequences and 5-prime RACE, assembled a full-length cDNA, which they designated PRAC (prostate, rectum, and colon), from LNCaP mRNA and prostate tumor total RNA. PRAC encodes a deduced 57-amino acid protein with several potential phosphorylation sites and a molecular mass of about 6 kD. RNA dot-blot analysis detected high expression of PRAC in prostate, rectum, and distal colon, and weak expression in bladder.
No difference in expression was found between normal prostate and prostate tumor samples. Northern blot analysis revealed a 450-bp PRAC transcript in prostate and colon as well as in the prostate adenocarcinoma cell lines LNCaP and PC-3. RT-PCR detected low expression in the prostate cell line DU145. Western blot analysis localized PRAC to the nucleus.
Organism species: Homo sapiens (Human)
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