Ring Finger Protein 148 (RNF148)

RNF148 is a novel chromatin-associated ubiquitin E3 ligase. It interacts and targets ubiquitinated H2A and H2AX, amplifying the local concentration of K63-linked ubiquitin conjugates, and assembles at DNA double-strand breaks in an RNF8-dependent manner.

The RING finger is a zinc-binding domain that is found in proteins from plants to humans, but whose function remains largely enigmatic. The domain itself is distinct from other zinc-finger motifs in terms of sequence homology, zinc-ligation scheme and three-dimensional structure. It appears that the RING is involved in mediating protein-protein interactions and in some cases multi-protein complexes, which might depend on the presence of other proteins and/or domains.