SAM And SH3 Domain Containing Protein 1 (SASH1)

The deduced 1,247-amino acid protein has a calculated molecular mass of about 140 kD. SASH1 contains 2 sterile alpha modules (SAMs) and an Src homology-3 (SH3) domain. These domains are predominantly found in signaling molecules, adaptors, and scaffold proteins.

SASH1 also has an N-terminal 25-residue proline-rich sequence. The mRNA has a long 3-prime untranslated region that has 2 polyadenylation signals. The human and mouse SASH1 proteins share 85% homology. Northern blot analysis detected a 4.4-kb transcript that was expressed ubiquitously, with highest levels in lung, placenta, spleen, and thymus. A 7.5-kb transcript was detected at lower levels in all tissues surveyed except brain.