Choline Transporter Like Protein 1 (CTL1)

Using an expression cloning approach, Wille et al. (2001) obtained a cDNA encoding CDw92. The deduced 652-amino acid CDw92 protein is 99% identical to the CTL1 protein reported by O'Regan et al. (2000). CDw92 has cytoplasmic N and C termini, 3 potential N-glycosylation sites, and a potential ITIM (immunoglobulin tyrosine-based inhibitory motif). Immunoprecipitation analysis showed that CDw92 is expressed as a 70-kD protein.

Flow cytometric analysis demonstrated expression of CDw92 on almost all B lymphocytes, most T lymphocytes, some NK cells, and some myeloid cell lines tested. Maturation of monocyte/dendritic cells resulted in downregulation of CDw92, and expression could subsequently be upregulated by interleukin-10 (IL10).