Active A Disintegrin And Metalloprotease 10 (ADAM10)

A Disintegrin and Metalloprotease 10 (ADAM10) is a membrane-anchored enzyme belonging to the ADAM family. It functions as a sheddase, cleaving the extracellular domains of numerous transmembrane proteins. This activity regulates key biological processes including cell adhesion, signaling, and proteolysis. ADAM10 is essential for embryonic development and is involved in the cleavage of substrates such as Notch, amyloid precursor protein (APP), and E-cadherin. Its function is critical for neural development, immune response, and tissue regeneration.Besides,A Disintegrin And Metalloprotease 17 (ADAM17) has been identified as an interactor of ADAM10, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ADAM10 and recombinant rat ADAM17. Briefly, ADAM10 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ADAM17-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADAM10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ADAM10 and recombinant rat ADAM17 was shown in Figure 1, the EC50 for this effect is 0.136 µg/mL.