Active Acid Phosphatase 1 (ACP1)
HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase
Overview
Properties
- Product No.APA994Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayHepatologyBone metabolism
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point6.7
Sign into your account
Share a new citation as an author
Upload your experimental result
Review

Contact us
Please fill in the blank.
Activity test

Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 ( assay buffer), initiated by addition 50 μl of 3 ug/ml ACP1 (diluted by assay buffer) to 50 µL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 μl of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/µg.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
-
BCA Protein Quantification Kit
-
Molecular Mass Marker for Protein
-
Monoclonal Antibody Customized Service
-
Polyclonal Antibody Customized Service
-
Protein Activity Test Experiment Service
-
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
-
Buffer
-
Lentivirus Packaging Experiment Service
-
Adenovirus Packaging Experiment Service
-
Real Time PCR Experimental Service
-
Spike RBD Protein (S-RBD)
-
Protein G
-
Protein A