Active Adrenomedullin (ADM) Mus musculus (Mouse) Active protein

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

No quotes Distributors
Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point10.1
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Active Adrenomedullin (ADM) Packages (Simulation)
  • Active Adrenomedullin (ADM) Packages (Simulation)
  • APA220Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Adrenomedullin (ADM) is a peptide hormone first discovered in 1993 in pheochromocytoma. Although AM is highly expressed in the adrenal glands, heart, lungs, and kidneys, vascular endothelium and smooth muscle are thought to be the main source of plasma AM. It is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. Receptor activity-modifying protein 2 can transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse ADM and recombinant human RAMP2. Briefly, ADM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAMP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse ADM and recombinant human RAMP2 was shown in Figure 1, the EC50 for this effect is 0.16 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2Wiley: Source
  • Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patientsPubmed: 23898869
  • Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.Pubmed: 23660671
  • DNA methylation patterns in newborns exposed to tobacco in uteroPubmed:25623364
  • Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and ProcalcitoninPubMed: 26271020
  • Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia.pubmed:28501895
  • Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
  • ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS:
  • Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failureDoi: 10.1016/j.phymed.2018.09.015
  • Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
  • Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life
  • Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497

Recommend products