Active Alpha-Fetoprotein (AFP) Homo sapiens (Human) Active protein

aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.5
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  • Active Alpha-Fetoprotein (AFP) Packages (Simulation)
  • Active Alpha-Fetoprotein (AFP) Packages (Simulation)
  • APA153Hu02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Alpha-Fetoprotein (AFP) ,as known as fetal alpha globulin or alpha-1-fetoprotein,is a glycoprotein belonging to the albumin family that is produced mainly by the fetal liver and yolk sac during pregnancy.Its primary function in the fetus is to transport calcium and fatty acids across the placenta, supporting fetal growth and development. In adults, elevated AFP levels can indicate certain medical conditions, including liver cancer and other diseases.Besides,Serpin A10 (SERPINA10) has been identified as an interactor of AFP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human AFP and recombinant human SERPINA10. Briefly, SERPINA10 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SERPINA10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. The binding activity of recombinant human AFP and recombinant human SERPINA10 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis modelPubMed: 20872761
  • Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda.Scirp: 5684
  • Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic ratsScienceDirect: S0168822711000520
  • Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liverScienceDirect: S0024320511002700
  • Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes.Hepatology: source
  • Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar ratsScienceDirect: S2210522013000348
  • Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention.Pubmed:25684529
  • Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometryRsc:Source
  • Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular CarcinomaPubmed:24927126
  • Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinomaPubMed: 25981587
  • Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinomaPubMed: 26643893
  • The concentrations of selected blood serum proteins in calves during the first three months of life85:33
  • Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion patternabstract:abstract4525.shtml
  • Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats2071_pdf.pdf
  • Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in ratsv04n07a07a
  • Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model1345-1349
  • Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathwaypubmed:28651490
  • Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticlesISSN 2451-2680
  • Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in ratspubmed:28720064
  • Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular CarcinomaPubmed:30045829
  • One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein10.1016:j.snb.2018.03.038
  • Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 andPubmed:29669302
  • Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel SegmentationPubmed:28107094
  • Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in RatsPubmed:29693337
  • The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular CarcinomaPubmed:29687726
  • Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer
  • Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine
  • The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKBPubmed: 30784932
  • Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma
  • Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrencePubmed: 31162867
  • Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in ratsPubmed: 31421311
  • Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transitionPubmed: 31652385
  • Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and …Pubmed: 32172678
  • The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian studyPubmed: 32198440
  • Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and InflammationPubmed: 32718197
  • Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles
  • Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS
  • Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
  • Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo
  • Blocking estrogen-induced AMH expression is crucial for normal follicle formation33658225
  • QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model34564023
  • In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC34698995

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