Active Annexin A1 (ANXA1) Rattus norvegicus (Rat) Active protein

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.3
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  • Active Annexin A1 (ANXA1) Packages (Simulation)
  • Active Annexin A1 (ANXA1) Packages (Simulation)
  • APE787Ra01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. To test the effect of ANXA1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant rat ANXA1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2h at 37oC. Proliferation of A549 cells after incubation with ANXA1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant rat ANXA1 for 48h. The result was shown in Figure 2. It was obvious that ANXA1 significantly inhibit cell viability of A549 cells. The ED50 is 1.75µg/mL.

Figure 2. Inhibition of A549 cells proliferation after stimulated with ANXA1.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns DiseasePubMed: PMC3794972
  • Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2Ajol: Source
  • Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic ResponseAsm: Source
  • Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)PubMed: 26718731
  • Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet FunctionPubMed: 26470781
  • Annexin A1 Is Increased in the Plasma of Preeclamptic WomenPubMed: 26398190
  • 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义article:8686
  • Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.pubmed:28062503
  • Mesenchymal stromal cells improve human islet function through released products and extracellular matrix.pubmed:29101297
  • Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesityPubmed:29677533
  • Annexin A1 is elevated in patients with COPD and affects lung fibroblast functionPubmed:29440885
  • Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patientsPubmed:29370415
  • EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT …pdf:3395
  • Resolution of inflammation in neuromyelitis optica spectrum disordersPubmed: 30300851
  • Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
  • Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolitesPubmed: 32133417
  • ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathwayPubmed: 32908567
  • Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients33263684
  • Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy33523920

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