Active Annexin A2 (ANXA2) Mus musculus (Mouse) Active protein

ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point7.6
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  • Active Annexin A2 (ANXA2) Packages (Simulation)
  • Active Annexin A2 (ANXA2) Packages (Simulation)
  • APB944Mu01.jpg Figure. SDS-PAGE
  • Active Annexin A2 (ANXA2) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of ANXA2 with S100A10.
Annexin A2 (ANXA2) also known as annexin II is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Besides, S100 Calcium Binding Protein A10 (S100A10) has been identified as an interactor of ANXA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA2 and recombinant mouse S100A10. Briefly, ANXA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A10-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA2 and S100A10 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosisPubMed: 20225235
  • Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinomaPubMed: PMC3491596
  • Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosisPubMed: PMC3742823
  • Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular CarcinomaGhrnet: Source
  • Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitisPubmed:24853056
  • Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variablesPubmed:25661364
  • Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis BPubMed: 26587036
  • Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinomaPubMed: 26109000
  • CirculatingPubMed: 25661364
  • Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinomaPubMed: 26189841
  • Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy populationPubMed: 26081791
  • Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma.pubmed:27889534
  • Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.pubmed:28501283
  • Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.pubmed:28181408
  • Expression of annexin A2 in adenomyosis and dysmenorrheaPubmed: 31183557
  • Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial CancerPubmed: 31842290
  • PLASMA BIOMARKER FOR OVARIAN CANCER
  • Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 6933406648
  • Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212

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