Active Brain Derived Neurotrophic Factor (BDNF) Rattus norvegicus (Rat) Active protein

Neurotrophin; Abrineurin

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point9.6
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  • Active Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • Active Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • APA011Ra01.png Figure. SDS-PAGE
  • Active Brain Derived Neurotrophic Factor (BDNF) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of BDNF with APP.
Brain-derived neurotrophic factor, also known as BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat BDNF and recombinant rat APP. Briefly, BDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of BDNF and APP was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810
  • Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.Pubmed:26559686
  • Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young ratspmc:PMC4812310
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
  • Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.pubmed:27914953
  • PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice.pubmed:27940378
  • Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalitiespubmed:28081639
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …bdi.12449
  • Associations of blood parameters with age, feed efficiency and sampling routine in young beef bullsscience:article
  • Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitispubmed:28412394
  • A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
  • Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal.pubmed:29042206
  • An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signalingPubmed:29627530
  • Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse modelPubmed:29335016
  • N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotypePubmed:29203442
  • Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemiaPubmed:30012511
  • Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model.Pubmed:29890692
  • Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley RatsPubmed:28933048
  • Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
  • Long term Westernized diet leads to region-specific changes in brain signaling mechanismsPubmed:29655945
  • Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclistsPubmed: 30286300
  • Establishment and evaluation of a novel mouse model of peri/postmenopausal depressionDoi: 10.1016/j.heliyon.2019.e01195
  • Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling
  • Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
  • Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute strokePubmed: 30826210
  • Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in micePubmed: 31077758
  • Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with DepressionPubmed: 30994834
  • Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model MicePubmed: 31561359
  • Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain SignalingPubmed: 31797207
  • Chronic harmine treatment has a delayed effect on mobility in control and socially defeated ratsPubmed: 32088835
  • Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicityPubmed: 32010279
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic childrenPubmed: 32327377
  • Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and …Pubmed: 32918393
  • Impaired LTD-like motor cortical plasticity in female patients with major depression disorderPubmed: 32791084
  • Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's diseasePubmed: 32777662
  • Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
  • Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety
  • Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …
  • Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine
  • Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke
  • Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi
  • Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­33389158
  • Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
  • Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 …34239303
  • Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy34586159
  • Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men34762211
  • Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression34824208
  • Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
  • Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid …34384732
  • CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway34296452
  • Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494
  • Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by …Pubmed:35344113
  • Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patientsPubmed:35508633
  • Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord …Pubmed:35214053
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377
  • Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder
  • Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress

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