Active Calcitonin Gene Related Peptide (CGRP) Rattus norvegicus (Rat) Active protein

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.4
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  • Active Calcitonin Gene Related Peptide (CGRP) Packages (Simulation)
  • Active Calcitonin Gene Related Peptide (CGRP) Packages (Simulation)
  • APA876Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Calcitonin gene-related peptide (CGRP) is a highly potent vasoactive peptide released from sensory nerves. CGRP is a 37-amino acid and it has two major forms (α and β). It belongs to a group of peptides that all act on an unusual receptor family. These receptors consist of calcitonin receptor-like receptor (CLR) linked to an essential receptor activity modifying protein (RAMP) that is necessary for full functionality. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CGRP and recombinant human RAMP2. Briefly, CGRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RAMP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CGRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CGRP and recombinant human RAMP2 was shown in Figure 1, the EC50 for this effect is 0.06 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo.Hindawi: 190370
  • Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological markerPubMed: PMC3620076
  • CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigraineOnlinelibrary: head.12372
  • Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid APubmed:Pmc4028234
  • Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot.Pubmed:24624298
  • Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyPubmed:24943060
  • OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migrainePubMed: 25735000
  • Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in ratsPubMed: 25892548
  • Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisPubmed:27156748
  • Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsPubmed:27250233
  • Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction.Pubmed:27144592
  • Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кBPubmed:27150105
  • Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition.pubmed:27445864
  • Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis.pubmed:27896372
  • A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
  • Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndromepubmed:28483500
  • CNS myeloid cells critically regulate heat hyperalgesiaPubmed:29634489
  • Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisalPubmed:30006780
  • RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis10.1101:357582
  • MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
  • Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migrainePubmed: 31070784
  • Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signalingPubmed: 31420791
  • Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …
  • Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455
  • Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion …Pubmed: 31375062
  • Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headachePubmed: 32286239
  • Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practicePubmed: 32951099
  • Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs33441852
  • Perirenal adipose afferent nerves sustain pathological high blood pressure in ratsPubmed:35668093

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