Active Calpain 2 (CAPN2) Homo sapiens (Human) Active protein

CANPL2; CANPml; mCANP; Calpain-2 Catalytic Subunit; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain large polypeptide L2; Millimolar-calpain; Calpain 2, Large Subunit

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.0
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  • Active Calpain 2 (CAPN2) Packages (Simulation)
  • Active Calpain 2 (CAPN2) Packages (Simulation)
  • APC358Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Calpain 2 (CAPN2) is a calcium-regulated, non-lysosomal thiolprotease that is known for its crucial role in catalyzing the limited proteolysis of substrates essential for cytoskeletal remodeling and signaling pathways. It belongs to the family of calpains, a group of enzymes that are activated by calcium ions.Besides,CAPN1 has been identified as an interactor of CAPN2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CAPN2 and recombinant rat CAPN1. Briefly, CAPN2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CAPN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humanCAPN2 and recombinant rat CAPN1 was shown in Figure 1, the EC50 for this effect is 0.044ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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