Active Carbonic Anhydrase III, Muscle Specific (CA3)

CA-III; CAIII; Car3; Carbonate dehydratase III

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 136.00 US$ 340.00 US$ 680.00 US$ 2,040.00 US$ 5,100.00
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Overview

Properties

Product No.APD070Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsCell culture; Activity Assays.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits Freeze-dried powder, Purity > 90%
Isoelectric Point7.4

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Packages (Simulation)
Packages (Simulation)

Figure. SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth. The activity of recombinant human CA3 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA3 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.