Active Carboxylesterase 1 (CES1)
ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1
- UOM
- FOB US$ 336.00 US$ 840.00 US$ 1,680.00 US$ 5,040.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.APC374Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayNeuro science
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 95%
- Isoelectric Point6.5
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Activity test

carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES1 (dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Spike RBD Protein (S-RBD)
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Protein A
Citations
- Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug InteractionsPubmed: 32764932