Active Cathepsin G (CTSG)

CG; CTS-G

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 216.00 US$ 540.00 US$ 1,080.00 US$ 3,240.00 US$ 8,100.00
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Overview

Properties

Product No.APD993Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsCell culture; Activity Assays.
Research use only
DownloadInstruction Manual
CategoryTumor immunity

Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits Freeze-dried powder, Purity > 95%
Isoelectric Point11.2

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Packages (Simulation)
Packages (Simulation)

Figure. SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Cathepsin G (CTSG) is a neutral proteinase secreted by neutrophil granulocytes. It belongs to the family of serine proteases. Cathepsin G is a glycoprotein, which contains 1% carbohydrate. The activity assay of recombinant rat CTSG was measured by its ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4 (Assay Buffer). The CTSG was diluted to different concentrations and the reaction was initiated by adding 50 ul CTSG to 50 ul 1 mM substrate and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is >70 pmol/min/µg.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

Increment services

Citations

Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.pubmed:28152632
Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer PrognosisPubmed: 31115649