Active Choline Acetyltransferase (ChAT)
CMS1A; CMS1A2; CHOACTase; Choline acetylase
Overview
Properties
- Product No.APB929Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayCardiovascular biologyNeuro science
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point8.4
Sign into your account
Share a new citation as an author
Upload your experimental result
Review

Contact us
Please fill in the blank.
Activity test

Choline Acetyltransferase (ChAT) is the enzyme responsible for synthesizing acetylcholine (ACh) from acetyl-CoA and choline in presynaptic neurons. It is crucial for cholinergic neurotransmission, influencing cognitive functions, muscle activation, and autonomic processes. ChAT is primarily localized in nerve terminals and is a biomarker for cholinergic neurons.ChAT synthesizes acetylcholine, while AChE breaks it down. They work in tandem to maintain the appropriate level of acetylcholine in the synaptic cleft for proper neural signaling.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human ChAT and recombinant mouse ChAT ACHE. Briefly, ChAT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACHE-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ChAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ChAT and recombinant mouse ChAT ACHE was shown in Figure 1, the EC50 for this effect is 0.127ug/mL.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
-
BCA Protein Quantification Kit
-
Molecular Mass Marker for Protein
-
Monoclonal Antibody Customized Service
-
Polyclonal Antibody Customized Service
-
Protein Activity Test Experiment Service
-
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
-
Buffer
-
Lentivirus Packaging Experiment Service
-
Adenovirus Packaging Experiment Service
-
Real Time PCR Experimental Service
-
Spike RBD Protein (S-RBD)
-
Protein G
-
Protein A
Citations
- Trans-resveratrol Inhibits Tau Phosphorylation in the Brains of Control and Cadmium Chloride-Treated Rats by Activating PP2A and PI3K/Akt Induced-Inhibition of …Pubmed: 30478674
- Melatonin Improves Memory Deficits in Rats with Cerebral Hypoperfusion, Possibly, Through Decreasing the Expression of Small-Conductance Ca2+-Activated K+ …Pubmed: 31187398
- Cerebrospinal fluid cholinergic biomarkers are associated with postoperative delirium in elderly patients undergoing Total hip/knee replacement: a …Pubmed: 32988385