Active Cluster Of Differentiation 28 (CD28)
Tp44; T-Cell-Specific Surface Glycoprotein
Overview
Properties
- Product No.APA652Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryCD & Adhesion molecule
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point6.2
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Activity test

The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown in Figure 1, the EC50 for this effect is 2.82 ug/mL.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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