Active Cluster Of Differentiation 36 (CD36)

The cluster of differentiation 36(CD36) is an 88 kDa transmembrane glycoprotein which is known also as SCARB3, member 3 (SR-B3)，fatty-acid translocator (FAT), glycoprotein 4 (GPIV) and PAS4. It is a member of a superfamily of scavenger receptor proteins class B, and functions as a receptor mediating the binding and chas multiple biological functions that may be important in inflammation and in the pathogenesis of metabolic diseases, including diabetes.It has been reported that THBS1 can mediate its antiangiogenic activity through CD36 to inhibit tumor angiogenesis, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD36 and recombinant rat THBS1. Briefly, biotin-linked CD36 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to THBS1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human CD36 and recombinant rat THBS1 was shown in Figure 1, the EC50 for this effect is 0.37ug/mL.